Journal: International Journal of Nanomedicine

Article Title: Human serum albumin-based probes for molecular targeting of macrophage scavenger receptors

doi: 10.2147/IJN.S197990

Figure Lengend Snippet: Evaluation of Mal-HSA-FITC uptake by different cell types. Fluorescence microscopy images of Mal-HSA-FITC probes (green) incubated for 3 h with ( A ) THP-1 macrophages, ( C ) HUVECs (EC), and ( D ) HCtSMCs (SMC). ( B ) Mal-HSA-FITC uptake by different cell types plotted against time. The results represent data from technical triplicates (9 images/well and timepoint) and are presented as mean and SEM. Data show significantly increased uptake of modified HSA probes by THP-1 cells compared with HUVEC (**** P <0.0001) and HCtSMCs ( #### P<0.0001) at 2.5 h and 3 h. None of the cell types recognized HSA-FITC (data not shown) (repeated measures two-way ANOVA (factors: cell type and time) and Tukey’s multiple comparison test). Abbreviations: HSA, human serum albumin; GCU, green calibration unit.

Article Snippet: Human carotid smooth muscle cells (HCtSMCs) (Cell Applications, San Diego, CA, USA) were cultured up to passage 7 in human smooth muscle cell media (Cell Applications).

Techniques: Fluorescence, Microscopy, Incubation, Modification, Comparison

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Article Title: Human Enterovirus in the Gastrocnemius of Patients With Peripheral Arterial Disease

doi: 10.1161/JAHA.113.000082

Figure Lengend Snippet: Viral RNA copy numbers were determined by RT‐qPCR. (A) Viral RNA copy numbers were compared among the Stage groups. (B) Single‐step growth curves of PAD‐HEV were examined at the various time points in the primary HSkm cells. Viral RNA copy numbers were determined using in vitro transcripts (see more detail in Supplemental Materials). PAD‐HEV1 (Stage III), PAD‐HEV2 (Stage II), PAD‐HEV3 and 4 (Stage IV). RT‐qPCR indicates reverse‐transcription real‐time quantitative polymerase chain reaction; PAD, peripheral arterial disease; HEV, human enterovirus; HSkm, primary human skeletal muscle; CVB1, coxsackievirus B1.

Article Snippet: HeLa cells (ATCC) were used for HEV propagation, HEV detection, and infectivity experiments., – Primary human skeletal muscle (HSkm) cells, cultured in MyoTonic Growth Medium (Cook MyoSite), also were used to study virus infectivity.

Techniques: Quantitative RT-PCR, In Vitro, Reverse Transcription, Real-time Polymerase Chain Reaction